How to use

step 1. Select the calculation mode

Calculation Mode

A. Solvation Free Energy (ΔG_solv)
B. Solvation Free Energy (ΔG_solv), Solvation Energy (ΔE_solv), and Solvation Entropy (TΔS_solv)
C. ΔG_solv + its residual decomposition
D. ΔG_solv, ΔE_solv, TΔS_solv + their residual decompositions

step 2. Choose an input PDB code

Protein source

You can find PDB codes at https://www.rcsb.org/

step 3. Submit and wait for the result

Please let us know your name, organization, email, and click submit button. Running calculation takes some time depending on protein size. In caase of Amyloid beta 42 (1IYT, 42 residues), solvation free energy calculation takes about 30 seconds and site directed analysis takes about 10 minutes. When calculation is done, server send you access link to your email.